Adsorption of protein on titanium dioxide and titanium dioxide coated surface
AbstractBiofunctions are required for metal implants introduced into stenotic blood vessels. These implants though required for dilation of the vessels, must exhibit blood compatibility and prevent the adhesion of platelets on its surface. Biomaterial grade titanium treated with polyethylene glycol as coating material was used for the experiment, with uncoated titanium as control. The adsorption of fibrinogen on both treated and untreated titanium oxide surfaces was studied. Whereas, there was significant adsorption of the fibrinogen protein on the untreated titanium oxide surface, none was recorded on the treated titanium oxide surface. Metal surface biofouling by proteins can be mitigated by the use polyethylene glycol coating on implants. This inhibition of proteins adsorption will prevent tissue growth, thereby allowing sliding movements in stenotic blood vessels. Key words: Biofouling, Fibrinogen, Polyethylene glycol, Titanium, Titanium oxide
P Tengvall; Proteins at Titanium Interfaces. In Titanium in Medicine, Brunette, D.M., Tengvall M. And
Thomson, P. eds., Berlin & Heidelberg :Springer-Verlag, 2001, 4457-484.
P Ratner; A Perspective on Biocompatibility. In Titanium in Medicine, Brunette, D.M., Tengvall, Textor M. and Thomson, P. eds,. Berlin and Heidelberg: Springer-Verlag. 2001, 1-12.
AJ Garcia; DM Collard; BG Keselowsky; SM Culter; ND Gallent; BA Byers; SN Stephansson; Engineering of Integrin-specific Biomimetic Surfaces. In Biomimetic Materials and design, Dillow, A.K. and Lowman, A.M., eds., New York-Bases: Marcel Dekker Inc., 2002, 29-55.
M Textor; S Tosatti; M Wieland; DM Brunette; Use of Molecular Assembly Techniques. In Bio-implant interface: Improving Biomaterials and Tissue Reaction, 2003, 341-346.
KE Healy; GM Harbers; TA Barber; DR Sumner; Bone Engineering, 2000, 268-281.
PA Cuypers; WT Hermens; HC Hemker; Analytical Biochemistry, 1978, 84 56-67.
N Lumbikanoda; R Sammons; International Journal of Oral Maxilofacial Implants, 2001, 16, 627-636.
JF Sundgren; P Bodo; B Ivarsson; I Lundstrom; Journal of Colloid Interface Science, 1986, 113, 538-543.
F Grinnell; MK Feld; Journal of Biomedical Materials Research, 1981, 15, 363-381.
F Grinnell; MK Feld; Journal of Biological Chemistry, 1982, 257, 4888-4893.