Optimization of alkaline protease production from Bacteria isolated from soil
AbstractProtease producing bacteria were isolated from soil and were identified as Pseudomonas fluorescens, Bacillus subtilus, E.coli and Serratia marscens. Optimization of the fermentation medium for maximum protease production was carried out. The culture conditions like pH, temperature, carbon sources and nitrogen sources were optimized. The Optimum conditions for protease production were found to be 37 o C at pH 10 with Glucose as Carbon Source and Peptone as Nitrogen Source. Soycake and Calcium chloride stimulates the production of protease with 1ml of inoculums size for 48 hrs of incubation period with less concentration of EDTA. Among all studied bacterial isolates, the highest enzyme activity was observed in B. Subtilis. Keywords: Alkaline protease, Soil, Optimization, Agrobased Waste material, Bacillus
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