Optimization of alkaline protease production from Bacteria isolated from soil

  • Pradeep Palsaniya Dr. B. Lal institute of Biotechnology, Malviya Industrial Area, Malviya Nagar, Jaipur
  • Rinki Mishra Dr. B. Lal institute of Biotechnology, Malviya Industrial Area, Malviya Nagar, Jaipur
  • Neha Beejawat Dr. B. Lal institute of Biotechnology, Malviya Industrial Area, Malviya Nagar, Jaipur
  • Sonia Sethi Dr. B. Lal institute of Biotechnology, Malviya Industrial Area, Malviya Nagar, Jaipur
  • B. Lal Gupta Dr. B. Lal institute of Biotechnology, Malviya Industrial Area, Malviya Nagar, Jaipur

Abstract

Protease producing bacteria were isolated from soil and were identified as Pseudomonas fluorescens, Bacillus subtilus, E.coli and Serratia marscens. Optimization of the fermentation medium for maximum protease production was carried out. The culture conditions like pH, temperature, carbon sources and nitrogen sources were optimized. The Optimum conditions for protease production were found to be 37 o C at pH 10 with Glucose as Carbon Source and Peptone as Nitrogen Source. Soycake and Calcium chloride stimulates the production of protease with 1ml of inoculums size for 48 hrs of incubation period with less concentration of EDTA. Among all studied bacterial isolates, the highest enzyme activity was observed in B. Subtilis. Keywords: Alkaline protease, Soil, Optimization, Agrobased Waste material, Bacillus

Author Biographies

Pradeep Palsaniya, Dr. B. Lal institute of Biotechnology, Malviya Industrial Area, Malviya Nagar, Jaipur
Dr. B. Lal institute of Biotechnology, Malviya Industrial Area, Malviya Nagar, Jaipur
Rinki Mishra, Dr. B. Lal institute of Biotechnology, Malviya Industrial Area, Malviya Nagar, Jaipur
Dr. B. Lal institute of Biotechnology, Malviya Industrial Area, Malviya Nagar, Jaipur
Neha Beejawat, Dr. B. Lal institute of Biotechnology, Malviya Industrial Area, Malviya Nagar, Jaipur
Dr. B. Lal institute of Biotechnology, Malviya Industrial Area, Malviya Nagar, Jaipur
Sonia Sethi, Dr. B. Lal institute of Biotechnology, Malviya Industrial Area, Malviya Nagar, Jaipur
Dr. B. Lal institute of Biotechnology, Malviya Industrial Area, Malviya Nagar, Jaipur
B. Lal Gupta, Dr. B. Lal institute of Biotechnology, Malviya Industrial Area, Malviya Nagar, Jaipur
Dr. B. Lal institute of Biotechnology, Malviya Industrial Area, Malviya Nagar, Jaipur

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Published
2017-03-31
Section
Article