Synthesis of silver nanoparticles and its effect on soil bacteria

  • J. Srivathsan Karpaga Vinayaga Institute of Medical Sciences and Research Centre, Kanchipuram, TN, India
  • V. Sivakami Department of Biotechnology, St. Joseph’s College of Engineering, Jeppiaar Nagar, Chennai, TN, India
  • B. Ramachandran Department of Biotechnology, St. Joseph’s College of Engineering, Jeppiaar Nagar, Chennai, TN, India
  • K. S. Harikrishna Karpaga Vinayaga Institute of Medical Sciences and Research Centre, Kanchipuram, TN, India
  • S. Vetriselvi Department of Microbiology, PGP College of Arts and Science, Namakkal, TN, India
  • D. J. Mukesh kumar Centre for Advanced Studies in Botany, Maraimalai Campus, University of Madras, Chennai, TN, India

Abstract

Silver Nanoparticles were synthesized from Silver nitrate with the help of metal reductant Trisodium citrate by Wet Chemical method. The antimicrobial activity of the Silver Nanoparticles was investigated against the Soil Bacteria isolated from rhizospheric regions through in vitro and in situ. In these tests, the agar plates were used and the Silver Nanoparticles of various concentrations were supplemented. As results, the Soil Bacteria were inhibited at the low concentration of silver Nanoparticles in both in vitro and in situ. These results suggest that the Silver Nanoparticles can be used as effective growth inhibitors in various bacteria, making them applicable to various antibacterial control systems. Keywords: Silver Nitrate, Wet Chemical synthesis, Antibacterial activity, In situ.

Author Biographies

J. Srivathsan, Karpaga Vinayaga Institute of Medical Sciences and Research Centre, Kanchipuram, TN, India
Karpaga Vinayaga Institute of Medical Sciences and Research Centre, Kanchipuram, TN, India
V. Sivakami, Department of Biotechnology, St. Joseph’s College of Engineering, Jeppiaar Nagar, Chennai, TN, India
Department of Biotechnology, St. Joseph’s College of Engineering, Jeppiaar Nagar, Chennai, TN, India
B. Ramachandran, Department of Biotechnology, St. Joseph’s College of Engineering, Jeppiaar Nagar, Chennai, TN, India
Department of Biotechnology, St. Joseph’s College of Engineering, Jeppiaar Nagar, Chennai, TN, India
K. S. Harikrishna, Karpaga Vinayaga Institute of Medical Sciences and Research Centre, Kanchipuram, TN, India
Karpaga Vinayaga Institute of Medical Sciences and Research Centre, Kanchipuram, TN, India
S. Vetriselvi, Department of Microbiology, PGP College of Arts and Science, Namakkal, TN, India
Department of Microbiology, PGP College of Arts and Science, Namakkal, TN, India
D. J. Mukesh kumar, Centre for Advanced Studies in Botany, Maraimalai Campus, University of Madras, Chennai, TN, India
Centre for Advanced Studies in Botany, Maraimalai Campus, University of Madras, Chennai, TN, India

References

F. Furno, K.S. Morley, B. Wong, B.L. Sharp, P.L. Arnold, S.M. Howdle, J. Antimicrob. Chemother., 2004, 54, 1019- 1024.

P.K. Stoimenov, R.L. Klinger, G.L. Marchin, K.J. Klabunde, Langmuir, 2002, 18, 6679–6686.

J.H. Crabtree, R.J. Burchette, R.A. Siddiqi, I.T. Huen, L.L. Handott, A. Fishman, Perit. Dial. Int., 2003, 23(4), 368- 374.

I. Sondi, B. Salopek-Sondi, J. Colloid Interface Sci., 2004, 275, 177 - 182.

J.S. Kim, E. Kuk, K.N. Yu, J-1. H. Kim, S.J. Park, H.J. Lee, S.H. Kim, Y.K. Park, Y.H. Park, C-Y. Hwang, Y-K. Kim, Y-S. Lee, D.H. Jeong, M-H. Cho, Nanomedicine: Nanotechnol. Biol. Medic., 2007, 3, 95–101.

I. Dragieva, S. Stoeva, P. Stoimenov, E. Pavlikianov, K. Klabunde, Nanostruct. Mater., 1999, 12, 267- 270.

Dušan Zvekić, V.S. Vladimir, A.K. Maja, N.M. Milan, Processing and Application of Ceramics, 2011, 5(1), 41–45

S. Silver, L.T. Phung, Annu. Rev. Microbiol., 1996, 50, 753- 789.

Ivan Sondi and Branka Salopek-Sondi, J.Colloid and Interface Science, 2004, 275, 177–182.

L. Brunet, D.Y. Lyon, E.M. Hotze, P.J.J. Alvarez, M. Wiesner, Environ. Sci. Technol., 2009, 43, 4355–4360.

M. Raffi, F. Hussain, T.M. Bhatti, J.I. Akhter, A. Hameed and M.M. Hasan, J. Mater. Sci. Technol., 2008, 24(2), 192-196.

Published
2017-03-31
Section
Article