Biological Hydrogen Production by using Immobilized Rhodobacter sphaeroides O.U 5

  • Kasoju Aruna JNTUA College of Engineering, Department of Biotechnology,Pulivendula-516390, Andhra Pradesh, India.
  • T. Mohammad Munawar JNTUA College of Engineering, Department of Biotechnology,Pulivendula-516390, Andhra Pradesh, India.

Abstract

Hydrogen is being considered by many scientists , as an ecofriendly and ideal fuel for the future, which can conveniently replace in many application .The aim of the study was to enhance photo production ofhydrogen by using immobilized Rhodobacter sphaeroides O.U 5 by optimizing a number of environmental and physical factors , like immobilization of various concentrations of salicylic acid , silica gel,cellulose,and various nitrogenous compounds. Immobilization of Rhodobacter sphaeroides O.U 5 , with 1% alginate , and with tubular physical geometry was shown to give maximum yield comparatively .Photoproduction of hydrogen was maximum with salicylic acid at a concentration of 0.02% w/v, and presence of silica was inhibited the hydrogen production compared to control . Higher concentrations of cellulose favour the hydrogen production. In general all the nitrogenous compounds tested , have been completely inhibited hydrogen photoproduction except in the presence of glutamate , where 50% production rate that of control was observed. Key words: Hydrogen, Immobilization, Rhodobacter sphaeroides O.U5, salicylic acid , silica gel.

Author Biographies

Kasoju Aruna, JNTUA College of Engineering, Department of Biotechnology,Pulivendula-516390, Andhra Pradesh, India.
JNTUA College of Engineering, Department of Biotechnology,Pulivendula-516390, Andhra Pradesh, India.
T. Mohammad Munawar, JNTUA College of Engineering, Department of Biotechnology,Pulivendula-516390, Andhra Pradesh, India.
JNTUA College of Engineering, Department of Biotechnology,Pulivendula-516390, Andhra Pradesh, India.

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Published
2017-03-31
Section
Article