Anti-mycobacterial activity of nanoparticles from Psidium guajava L.
AbstractThe leaves of Psidium guajava L.used in present investigation were collected from Nanded district of Maharashtra India. Non pathogenic strains used in this study are Mycobacterium tuberculosis, M. spegmatis, M.pheli were obtained from, Institute of Microbial Technology, Chandigarh (PB) India. All the strains were subcultured and maintained on Lowenstein Jensen Media. The plant material was ground in the mortar and pestle and extracted with methanol. The plant extract were added with 1 mM AgNO3 and 1mM CuCl2 and incubated for the synthesis of plant based silver and copper nano particles. It was confirmed by UV-visible spectroscopy. After that the anti- Mycobacterial activity was determined by disc diffusion method. Anti-Mycobacterial discs were prepared by dipping them in plant base nano particles of 1 mM AgNO3 and 1mM CuCl2. Five different concentrations such as 100μl, 200μl, 300μl, 400μl, 500μl of plant based nano particles extract were used respectively. The Anti-Mycobacterial activity of particles from Psidium guajava L. showed both silver (14.23±0.69) and copper (13.13±0.19) nanoparticles positive activity. It was observed that plant based silver nanoparticles showed highest activity than copper nanoparticles. The activity depends on the concentration of nanoparticles in plant extract. Key words: Silver and copper nano particles, Non pathogenic bacteria, Psidium guajava Anti-Mycobacterial activity.
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