Studies on the free radical scavenging activity of Calamus prasinus

  • H. N. Krishna Kumar Department of Studies in Biotechnology, Microbiology and Biochemistry, Pooja Bhagavat Memorial Mahajana Post Graduate Centre, Affiliated to University of Mysore, Metagalli, Mysore, Karnataka, India
  • Jyoti Bala Chauhan Department of Studies in Biotechnology, Microbiology and Biochemistry, Pooja Bhagavat Memorial Mahajana Post Graduate Centre, Affiliated to University of Mysore, Metagalli, Mysore, Karnataka, India

Abstract

In the present study, the antioxidant potentials of aqueous and methanolic extracts of Calamus prasinus fruits were investigated. The antioxidant activity was assessed through DPPH assay, superoxide anion radical scavenging assay and reducing power assay. In vitro antioxidant activity of the plant extract revealed that both the extracts showed good antioxidant power with IC50 values of 25μg/mL and 32μg/mL in DPPH assay for methanolic extract and aqueous extract respectively which is almost comparable to standard antioxidant ascorbic acid (IC50-5μg/mL). The reducing power of the extract was found to be concentration dependent. The study showed, both methanolic and aqueous extracts were found to possess scavenging effects on superoxide anions at concentrations dependent manner with an IC 50 value of 104 and 126 μg/ml respectively. The total phenolic content was determined by Folin- Ciocalteu method. Both the extracts showed the highest total phenolics i.e., 88.2mg/g (aqueous extract) and 55.4mg/g (methanolic extract) equivalent weight of gallic acid. Key words: Antioxidant activity, Reducing power, Polyphenolics, Free radicals

Author Biographies

H. N. Krishna Kumar, Department of Studies in Biotechnology, Microbiology and Biochemistry, Pooja Bhagavat Memorial Mahajana Post Graduate Centre, Affiliated to University of Mysore, Metagalli, Mysore, Karnataka, India
Department of Studies in Biotechnology, Microbiology and Biochemistry, Pooja Bhagavat Memorial Mahajana Post Graduate Centre, Affiliated to University of Mysore, Metagalli,Mysore, Karnataka, India
Jyoti Bala Chauhan, Department of Studies in Biotechnology, Microbiology and Biochemistry, Pooja Bhagavat Memorial Mahajana Post Graduate Centre, Affiliated to University of Mysore, Metagalli, Mysore, Karnataka, India
Department of Studies in Biotechnology, Microbiology and Biochemistry, Pooja Bhagavat Memorial Mahajana Post Graduate Centre, Affiliated to University of Mysore, Metagalli,Mysore, Karnataka, India

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Published
2017-03-27
Section
Article