Prevalence of blaTEM AND blaSHV genes in clinical Isolates of Klebsiella pneumoniae in a tertiary care hospital
AbstractThis study was carried out to determine the existence and to describe phenotypic and genotypic characteristics of blaTEM and blaSHV genes in extended-spectrum β-lactamase (ESBL) producing Klebsiellapneumoniae. The study was also aimed to compare results of PCDDT and genotypic methods. Information on molecular types of ESBL positive Klebsiellapneumoniae is less from India ; lesser still from this area of Marathwada , a part of Maharashtra state . We conducted this study to look for these genes in ESBL positive Klebsiellapneumoniae isolated from the patients from Govt. Medical College and Hospital , Aurangabad. A total of 300 strains of Klebsiella pneumoniae were selected for the study from Jan 2013- June 2013. Kirby – Bauer disk diffusion method was performed to determine the antibiotic resistance pattern. Screened for ESBL and confirmed by phenotypic confirmatory disc diffusion test (PCDDT). 100 randomly selected isolates were investigated for the presence of blaTEM and blaSHV genes via Polymerase chain reaction (PCR) .Multiplex PCR was also performed for the same. Phenotypic confirmatory test was able to detect ESBL production in 90.07% of Klebsiella pneumoniae isolates. Among the two ESBL genotypes, the most prevalent genotype was found to be blaTEM. Majority of ESBL producing isolates possess both ESBL genes. Multiplex PCR can be used as a rapid method to identify common genes (blaTEM and blaSHV) responsible for extended spectrum beta lactamase production in Klebsiella pneumoniae.Saving precious time and resources, more importantly early treatment. PCDDT results correlated with genotypic method in all the tested strains. Key words: ESBL, Klebsiella pneumoniae, blaTEM,blaSHV.
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