Study the modern biochemical analysis techniques of proteins and alkaline phasphtase enzyme system from biological sample chicken liver
AbstractThe objective of the study was the biochemical analysis of proteins and Alkaline Phosphatase enzyme system from biological sample Chicken Liver using modern biochemical analysis techniques, including protein extraction, fractionation and electrophoresis separation technique and enzyme analysis. The amino acids ia polypeptide chain are linked by peptide bonds. Once linked in the protein chain, an individual amino acid is called a residue, and the linked series of carbon, nitrogen and oxygen atoms are known as the main chain are protein backbone .The end of the protein with a free carboxyl group is known as the C-terminus or carboxyl terminus, whereas the end with a free amino group is known as the N-terminus or amino terminus. Keywords: Proteins, Amino acids, Protein chains, Biochemical analysis, Enzymes etc.
S.Miyazaki, Y.Motoi. British Poultry Science, 1466-1799, Volume 37, Issue 3, 1996, Pages 651 – 660
Lowry et al. J. Biol. Chem. 193: 265-275 (1951)
Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 k Da Anal Biochem 1987.
Bishop, D., Roche, C., and Nisman, B. Biochem J 90, 378, 1964.
Chernecky CC, Berger BJ (2008). Laboratory Tests and Diagnostic Procedures, 5th ed. St. Louis: Saunders.
Fischbach FT, Dunning MB III, eds. (2009). Manual of Laboratory and Diagnostic Tests, 8th ed. Philadelphia: Lippincott Williams and Wilkins.
Pagana KD, Pagana TJ (2006). Mosby’s Manual of Diagnostic and Laboratory Tests, 3rd ed. St. Louis: Mosby.