Antimicrobial activity and Phytochemical screening of the medicinal plant Heliotropium indicum L.
AbstractAqueous and chloroform extracts of Heliotropium indicum were screened for phyto chemicals and antimicrobical activity against seven bacterial organism Klebsiella pneumoniae , Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogens, Serratia marcescens, Pseudomonas aeruginosa and Escherichia coli and five fungal organisms Aspergillus niger , Aspergillus fumigatus, Ganoderma lucida, Mucor indicus and Saccharomyces cervisiae by well diffusion method. The phytochemical analyses showed the presence of saponin, tannin, alkaloid, phenol and proteins. Among the two extracts assessed aqueous extract had significant inhibiting activity than chloroform extract. The maximum zone of inhibition was recorded Escherichia coli in bacterial strains and Ganoderma lucida was recorded in fungal strains. Key Words: , Heliotropium indicum, Phytochemical, Antimicrobial activity.
Farnsworth, N.R. Akerele, O.,Bingel, A.S., Soejarto, D.D. and Guo, Z. 1985. Bull. Of WHO., 63:965-981.
Essawi T and Srour M, 2000. J.Ethnopharmacol., 70:343-349.
Woodford N, 2003. Expert Opinion Investigation and Drugs, 12:117.
Nadkarni, K.M. 1976. Indian Materia Medica, Vol. I: p. 972. Popular Prakashan Pvt. Limited, Bombay.
Kirtikar, K.R., and Basu, D.D. 1975. Indian Medicinal Plants. Vol-I, II and III. Bishen Singh Mahendra Pal Singh, CSIR. New Delhi.
Gamble, J.S. 1935. Flora of the Presidency of Madras, I-III,Botanical Survey of India, Cultutta.
Mathew, K.M. 1983. The Flora of Tamilnadu and Carnatic., The Rapinat Herbarium, St. Joseph, College, Tiruchirapalli, India.
Henry, A.N., Kumari G.R and Chithra, V. 1987. Flora of Tamil Nadu, India, Series 1: Vol. 2: Botanical Survey of India, Southern Circle, Coimbatore.
Anonymous. 1966. Indian Pharamacopoeia, 2nd Edn. Government of India, New Delhi.
Kokate, C.K., Khandewal, K.R., Pawar, A.P and Gohale, S.B. 1995. Practical Pharmacognosy, 3rd edn., Nirali Prakashan, Pune.
Bauer, A.W., Kirby W.M.M. Truck, H and Shreeies, J.C. 1996. Amer. J. Clin. Pathol., 45:493-496
NCCLS 2001. National Committee for Clinical Laboratory Standards, Performance standards for Antimicrobial susceptibility testing. Eleventh informational supplemented. M 100- 311, Way ne, PA, USA.
NCCLS 2004. National Committee for Clinical Laboratory Standards, Performance standards for Antimicrobial susceptibility testing. Fourteenth informational supplemented. M 100- S14, Way ne, PA, USA.
Sofowora A, 1986. Medicinal plant and traditional medicine in Africa II, p.178. John Wiley Chiechester.
Ebana R.U.B, Madunagu B. E, Ekpe E.D and Otung A. 1991. J.Appli Bacteriol., 71:398-401.
Cushnie TP, Lamb AJ, 2005. Int. J. Antimicrobial Agents, 26: 343.
Tsuchiya, H., Sato, M., Fugiwara, S.,Tanigaki, S., Chyama, M., Tanaka, T and Linuma, M (1996). Ethnopharmacol., 50:27-34.
Singh, J.P., Pandy, D.P., Pandey, M.B. Singh, A. and Singh, R. 2005. J. Ind. Chem. Soc., 82(2): 175-176.
De. N., Maori. L. and Ardo. H. 2009. Journal of Medicinal plants Rsearch., Vol.(3). pp. 116-119.