Characterization and applications of Pullulan and chitosan produced by fermentation
AbstractChitin is highly insoluble N - acetylated polymer yet to find large scale industrial applications. Chitosan is an acid soluble deacetylated form of chitin. The physicochemical characteristics of chitosan depend on the degree of deacetylation. In this research work cell wall pullulan, an alpha glucan was isolated from the strain Aureobasidium mausonii NCIM 1226 the biomass obtained was further transformed in to another useful polymer chitosan using the strain Alcaligenes faecalis NCIM 2444. Produced polymers were characterized by using FT-IR, and 1HNMR spectroscopic methods. The polymers were also evaluated for its antimicrobial activity. Chitosan possessed degree of deacetylation (DDA) in the range of 86-91%, and both standard and isolated polymers having identical FT-IR, 1HNMR spectrum. Chitosan possesses good antibacterial activity and pullulan showed negative antimicrobial activity. Keywords: Chitin, chitosan, pullulan, degree of deacetylation, microspheres
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