A comparative study of lipid profiles of cyanobacteria from two biodiversity hotspots of India (Indo-Burma and Western Ghats)

  • Avijeet Singh Oinam Freshwater Cyanobacterial and Microalgal Repository, Microbial Resources Division, Institute of Bioresources and Sustainable Development (IBSD)(A National Institute of Department of Biotechnology, Govt. of India) Takyelpat, Imphal West, Manipur, India
  • Ojit Singh Keithellakpam Freshwater Cyanobacterial and Microalgal Repository, Microbial Resources Division, Institute of Bioresources and Sustainable Development (IBSD)(A National Institute of Department of Biotechnology, Govt. of India) Takyelpat, Imphal West, Manipur, India
  • Silvia Chungkham Freshwater Cyanobacterial and Microalgal Repository, Microbial Resources Division, Institute of Bioresources and Sustainable Development (IBSD)(A National Institute of Department of Biotechnology, Govt. of India) Takyelpat, Imphal West, Manipur, India
  • Indrama Thingujam Freshwater Cyanobacterial and Microalgal Repository, Microbial Resources Division, Institute of Bioresources and Sustainable Development (IBSD)(A National Institute of Department of Biotechnology, Govt. of India) Takyelpat, Imphal West, Manipur, India
  • Gunapati Oinam Freshwater Cyanobacterial and Microalgal Repository, Microbial Resources Division, Institute of Bioresources and Sustainable Development (IBSD)(A National Institute of Department of Biotechnology, Govt. of India) Takyelpat, Imphal West, Manipur, India
  • Romi Khangembam Freshwater Cyanobacterial and Microalgal Repository, Microbial Resources Division, Institute of Bioresources and Sustainable Development (IBSD)(A National Institute of Department of Biotechnology, Govt. of India) Takyelpat, Imphal West, Manipur, India
  • Thadoi Devi Angom Freshwater Cyanobacterial and Microalgal Repository, Microbial Resources Division, Institute of Bioresources and Sustainable Development (IBSD)(A National Institute of Department of Biotechnology, Govt. of India) Takyelpat, Imphal West, Manipur, India
  • Indira Devi Wangkhem Freshwater Cyanobacterial and Microalgal Repository, Microbial Resources Division, Institute of Bioresources and Sustainable Development (IBSD)(A National Institute of Department of Biotechnology, Govt. of India) Takyelpat, Imphal West, Manipur, India
  • Minerva Shamjetshabam Freshwater Cyanobacterial and Microalgal Repository, Microbial Resources Division, Institute of Bioresources and Sustainable Development (IBSD)(A National Institute of Department of Biotechnology, Govt. of India) Takyelpat, Imphal West, Manipur, India
  • Subhalaxmi Sharma Aribam Freshwater Cyanobacterial and Microalgal Repository, Microbial Resources Division, Institute of Bioresources and Sustainable Development (IBSD)(A National Institute of Department of Biotechnology, Govt. of India) Takyelpat, Imphal West, Manipur, India
  • Onkar Nath Tiwari Freshwater Cyanobacterial and Microalgal Repository, Microbial Resources Division, Institute of Bioresources and Sustainable Development (IBSD)(A National Institute of Department of Biotechnology, Govt. of India) Takyelpat, Imphal West, Manipur, India
  • Gauri Dutt Sharma Department of Life Sciences and Bioinformatics, Hargobind Khurana School of Life Sciences, Assam University, Silchar, Assam, India

Abstract

Five non-heterocystous filamentous cyanobacteria were isolated from Indo-Burma Biodiversity Hotspot namely, Phormidium animale BTA 258 (P1), Leptolyngbya sp. BTA 287 (L1), Leptolyngbya sp. BTA 477 (L2), Leptolyngbya tenerrima BTA 766 (L3) and Phormidium tenue BTA 803 (P2). Fatty acids were profiled and compared with cyanobacterial fatty acids reported from Western Ghats. Cyanobacterial SFA% and MUFA% of Indo-Burma region were comparatively less as compared to Western Ghats with highest shown by heterocystous cyanobacteria (41- 98% total fatty acids). Indo Burma cyanobacterial isolates present more MUFA% in average as compared to PUFA%. PUFA% were high in P1 (11.20%) and P2 (11.36%) compared to non-heterocystous groups of both the hotspot. Butyric acid (C4:0) was significant as specific chemotaxonomic marker for the Phormidium animale and Leptolyngbya sp. of Indo-Burma region. High amount of C13:0, C14:0, C14:1, C15:0, C20:1 and C20:2 were obtained in Phormidium and Leptolyngbya of Indo-Burma region with C13:0 and C14:1 being the highest and present specifically. C15:0, C18:2n6t, C20:3n6, C22:1n9, C20:3n3, C20:4n6, C20:5n3, C22:2 and C24:1 showed diversed distribution and strain dependent at both the regions. C16:0 was found commonly in all the cyanobacteria of both the hotspots. C14:1, C15:1, C16:1, C17:1 and C18:1nt were absent in Phormidium strains of Western Ghats but present in both the Phormidium strains of Indo Burma region. Heterocystous strains of Western Ghats showed high GLA (C18:3n6) content compared to isolates of Indo-Burma region. Potential cyanobacterial candidates for chemotaxonomic marker and value added products from two hotspots of India can be ruled out from this current investigation. Keywords: Comparison, Cyanobacteria, fatty acids, Indo-Burma, Western Ghats

Author Biographies

Avijeet Singh Oinam, Freshwater Cyanobacterial and Microalgal Repository, Microbial Resources Division, Institute of Bioresources and Sustainable Development (IBSD)(A National Institute of Department of Biotechnology, Govt. of India) Takyelpat, Imphal West, Manipur, India
Freshwater Cyanobacterial and Microalgal Repository, Microbial Resources Division, Institute of Bioresources and Sustainable Development (IBSD)(A National Institute of Department of Biotechnology, Govt. of India) Takyelpat, Imphal West, Manipur, India
Ojit Singh Keithellakpam, Freshwater Cyanobacterial and Microalgal Repository, Microbial Resources Division, Institute of Bioresources and Sustainable Development (IBSD)(A National Institute of Department of Biotechnology, Govt. of India) Takyelpat, Imphal West, Manipur, India
Freshwater Cyanobacterial and Microalgal Repository, Microbial Resources Division, Institute of Bioresources and Sustainable Development (IBSD)(A National Institute of Department of Biotechnology, Govt. of India) Takyelpat, Imphal West, Manipur, India
Silvia Chungkham, Freshwater Cyanobacterial and Microalgal Repository, Microbial Resources Division, Institute of Bioresources and Sustainable Development (IBSD)(A National Institute of Department of Biotechnology, Govt. of India) Takyelpat, Imphal West, Manipur, India
Freshwater Cyanobacterial and Microalgal Repository, Microbial Resources Division, Institute of Bioresources and Sustainable Development (IBSD)(A National Institute of Department of Biotechnology, Govt. of India) Takyelpat, Imphal West, Manipur, India
Indrama Thingujam, Freshwater Cyanobacterial and Microalgal Repository, Microbial Resources Division, Institute of Bioresources and Sustainable Development (IBSD)(A National Institute of Department of Biotechnology, Govt. of India) Takyelpat, Imphal West, Manipur, India
Freshwater Cyanobacterial and Microalgal Repository, Microbial Resources Division, Institute of Bioresources and Sustainable Development (IBSD)(A National Institute of Department of Biotechnology, Govt. of India) Takyelpat, Imphal West, Manipur, India
Gunapati Oinam, Freshwater Cyanobacterial and Microalgal Repository, Microbial Resources Division, Institute of Bioresources and Sustainable Development (IBSD)(A National Institute of Department of Biotechnology, Govt. of India) Takyelpat, Imphal West, Manipur, India
Freshwater Cyanobacterial and Microalgal Repository, Microbial Resources Division, Institute of Bioresources and Sustainable Development (IBSD)(A National Institute of Department of Biotechnology, Govt. of India) Takyelpat, Imphal West, Manipur, India
Romi Khangembam, Freshwater Cyanobacterial and Microalgal Repository, Microbial Resources Division, Institute of Bioresources and Sustainable Development (IBSD)(A National Institute of Department of Biotechnology, Govt. of India) Takyelpat, Imphal West, Manipur, India
Freshwater Cyanobacterial and Microalgal Repository, Microbial Resources Division, Institute of Bioresources and Sustainable Development (IBSD)(A National Institute of Department of Biotechnology, Govt. of India) Takyelpat, Imphal West, Manipur, India
Thadoi Devi Angom, Freshwater Cyanobacterial and Microalgal Repository, Microbial Resources Division, Institute of Bioresources and Sustainable Development (IBSD)(A National Institute of Department of Biotechnology, Govt. of India) Takyelpat, Imphal West, Manipur, India
Freshwater Cyanobacterial and Microalgal Repository, Microbial Resources Division, Institute of Bioresources and Sustainable Development (IBSD)(A National Institute of Department of Biotechnology, Govt. of India) Takyelpat, Imphal West, Manipur, India
Indira Devi Wangkhem, Freshwater Cyanobacterial and Microalgal Repository, Microbial Resources Division, Institute of Bioresources and Sustainable Development (IBSD)(A National Institute of Department of Biotechnology, Govt. of India) Takyelpat, Imphal West, Manipur, India
Freshwater Cyanobacterial and Microalgal Repository, Microbial Resources Division, Institute of Bioresources and Sustainable Development (IBSD)(A National Institute of Department of Biotechnology, Govt. of India) Takyelpat, Imphal West, Manipur, India
Minerva Shamjetshabam, Freshwater Cyanobacterial and Microalgal Repository, Microbial Resources Division, Institute of Bioresources and Sustainable Development (IBSD)(A National Institute of Department of Biotechnology, Govt. of India) Takyelpat, Imphal West, Manipur, India
Freshwater Cyanobacterial and Microalgal Repository, Microbial Resources Division, Institute of Bioresources and Sustainable Development (IBSD)(A National Institute of Department of Biotechnology, Govt. of India) Takyelpat, Imphal West, Manipur, India
Subhalaxmi Sharma Aribam, Freshwater Cyanobacterial and Microalgal Repository, Microbial Resources Division, Institute of Bioresources and Sustainable Development (IBSD)(A National Institute of Department of Biotechnology, Govt. of India) Takyelpat, Imphal West, Manipur, India
Freshwater Cyanobacterial and Microalgal Repository, Microbial Resources Division, Institute of Bioresources and Sustainable Development (IBSD)(A National Institute of Department of Biotechnology, Govt. of India) Takyelpat, Imphal West, Manipur, India
Onkar Nath Tiwari, Freshwater Cyanobacterial and Microalgal Repository, Microbial Resources Division, Institute of Bioresources and Sustainable Development (IBSD)(A National Institute of Department of Biotechnology, Govt. of India) Takyelpat, Imphal West, Manipur, India
Freshwater Cyanobacterial and Microalgal Repository, Microbial Resources Division, Institute of Bioresources and Sustainable Development (IBSD)(A National Institute of Department of Biotechnology, Govt. of India) Takyelpat, Imphal West, Manipur, India
Gauri Dutt Sharma, Department of Life Sciences and Bioinformatics, Hargobind Khurana School of Life Sciences, Assam University, Silchar, Assam, India
Department of Life Sciences and Bioinformatics, Hargobind Khurana School of Life Sciences, Assam University, Silchar, Assam, India

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Published
2017-03-31
Section
Article