Evaluation of antibacterial and antifungal activity of macro algae associated microbes from the Northern and Southern coast of Kenya

  • Purity K. Kaaria Department of Botany, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya
  • Viviene N. Matiru Department of Botany, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya
  • Joseph G. Wakibia Department of Botany, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya
  • Mary W. Ndung’u Department of Chemistry, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya
  • Christine C. Bii Kenya Medical Research Institute-Centre for Microbiology, Nairobi, Kenya

Abstract

Marine macro algae are known to be an excellent source of bioactive compounds which have a broad range of biological activities including antibacterial and antioxidant. The surfaces of marine eukaryotes provide a unique habitat for colonizing microorganisms which also produce bioactive compounds. The Kenyan Indian Ocean is a host of numerous seaweeds species. The present study embarked on isolation of microbes that live in association with the macro algae collected from three sites in the Northern (Mtwapa and Mkomani) and Southern coast (Kibuyuni) of Kenya during the Southeast monsoon season. Microbes were isolated from different species of the red, green and brown macro algae and evaluated for their antibacterial and antifungal activity, against three human pathogens namely; Gram positive Staphylococcus aureus (ATCC 25922), Gram negative Escherichia Coli (ATCC 25923) and fungi Candida albicans (ATCC 90028). The study obtained 1609 microbes and 187 isolates showed inhibition potential against the test organisms, with most isolates (91) showing good activity against Escherichia Coli followed by Candida albicans (78 isolates) and Staphylococcus aureus (49 isolates) respectively. From the results, it clearly depicts the potential of the macro algae associated microbes. This means that the marine microbes may be useful in the treatment of various infectious caused by different pathogens. Key words: Antimicrobial potential, Bioactive compounds, Test organisms, Seaweeds,

Author Biographies

Purity K. Kaaria, Department of Botany, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya
Department of Botany, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya
Viviene N. Matiru, Department of Botany, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya
Department of Botany, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya
Joseph G. Wakibia, Department of Botany, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya
Department of Botany, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya
Mary W. Ndung’u, Department of Chemistry, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya
Department of Chemistry, Jomo Kenyatta University of Agriculture and Technology, Nairobi, Kenya
Christine C. Bii, Kenya Medical Research Institute-Centre for Microbiology, Nairobi, Kenya
Kenya Medical Research Institute-Centre for Microbiology, Nairobi, Kenya

References

J. W. Blunt; B. R. Copp; M. H. G. Munro; P. T. Northcote and M. R. Prinsep. Nat. Prod. Rep. 2004. 21(1), 1–49.

L. Zhiyong. Mar. Drugs. 2009. 7, 113-129.

National Committee for Clinical Laboratory Standards Performance standards for antimicrobial disc susceptibility test 8th edition. 2003, M2-A8, NCCLS, Wayne, PA.

E. Armstrong; A. Rogerson; J.W. Leftley. Estuar Coast Shelf Sci. 2000. 50, 415-424.

V. Janakidevi; M. YokeshBabu; R. Umarani; A.K. Kumaraguru. Int J Curr Microbiol App Sci. 2013. 2 (12), 140-147.

G. Strobel. Microbes Infect. 2003. 5 (6), 535-544.

M. Kanagasabhapathy; H. Sasaki; S. Nagata. World J Microbiol Biotechnol. 2008. 24, 2315–2321.

S. Vijayalakshmi; M. S. Ramasamy; S. Murugesh, A. Murugan. Annals of Microbiology. 2008. 58 (4), 605-609.

S.W. George; A.R. David; L.C. Shannon; V.N. Sham. Microbiological Research. 2009. 165 (2010), 300—311

Y. Zhang; M. Jun; F. Yan; Y. Kang; J. Zhang; G. Peng-Juan, Y.Wang; M. Li-Fang; Z. Yan-Hua. Mar. Drugs. 2009. 7, 97-112.

L.J. Villarreal-Gómez; E. Irma; Soria-Mercado, G. Guerra-Rivas and E. Nahara; Ayala-Sánchez. Revista de Biología Marina y Oceanografía. 2010. 45 (2), 267-275.

S. Ragil; S. Agus, W. Ita. Procedia Environmental Sciences. 2014. 23 (2015), 240 – 246.

E.A. Paz; R.N. Lacy; M. Bakhtian. The β- Lactum antibiotics penicillin and cephalosporin in perspective. Hodder strong, London. 1995. pp. 324.

G.J. Tortora; B.R. Funke. C.L. Case. In Microbiology. An Introduction. Benjamin Cummings, San Francisco, 2001, pp. 88

T.E.Tshikalange; J. J. M.Meyer; A.A. Hussein. Journal of Ethnopharmacology. 2005. 96, 515-519.

C. Ibtissam; R. Hassane; J. Martinez-Lopez; J.F. Dominguez Seglar; J.A. Gomez Vidal; B. Hassan; K. Mohamed. 2009. 8(7), 1258-1262.

J.P. Marechal; G. Culioli; C. Hellio; H.Thomas-Guyon; M.E. Callow; A.S. Clare; A. Ortalo-Magné. J. Exp.

Mar. Biol. Ecol. 2004. 313, 47-62.

N. Salvador; A. Gomez-Garreta; L. Lavelli; M.A. Ribera. Scientia Marina. 2007. 71, 101-114.

Y. Freile-Pelegrin; J.L. Morales. Botanica Marina. 2004. 47(2), 140-146.

K. Padmakumar; K. Ayyakkannu. Botanica Marina. 1997. 40: 507-515.

K. Kolanjinathan; P. Ganesh; M. Govindarajan. European Review for Medical and Pharmacological Sciences. 2009. 13,173-177.

K.Vallinayagam; R. Arumugam; R. R. K. Ragupathi; G. Thirumaran; P. Anantharaman. Global Journal of Pharmacology. 2009. 3(1), 50-52.

M. Kandhasamy; K.D. Arunachalam. Afr. J. Biotechnol. 2008. 7(12), 1958-1961.

C. Saranya; C. Parthiban; P. Anantharaman. Advances in Applied Science Research. 2014. 5(4), 82-90

Published
2017-03-28
Section
Article