Biosynthesis of silver nanoparticles (AgNPs) using different plants and fruits extract and study their antimicrobial activity

  • Sandeep Thapa College for Professional Studies, Department of Biotechnology, Kathmandu, Nepal
  • Rajani Shresth College for Professional Studies, Department of Biotechnology, Kathmandu, Nepal
  • Anjali Tibrewal College for Professional Studies, Department of Biotechnology, Kathmandu, Nepal
  • Mukesh Shrestha College for Professional Studies, Department of Biotechnology, Kathmandu, Nepal
  • Yuvraj K. C. College for Professional Studies, Department of Biotechnology, Kathmandu, Nepal

Abstract

The modern era of Nanotechnology has emerged with the biosynthesis of nanoparticles which can be used aspotential antibiotics for multidrug resistance bacteria, the major health issues of the world. This study emphasis on simple, economical and eco-friendly method for biosynthesis of silver nanoparticles using different plants and fruits extract. A total of five samples, leaves of Nyctanthes arbor-tristis, Moringa oleifera, Zanthoxylum armatum and fruits of Choerospondias axillaris and Areca catechu were selected for the biosynthesis of Silver nanoparticles and study of their respective antimicrobial activity. The samples were sterilized, peeled off, grinded and followed by boiling with distilled water for 20 minutes. The aqueous extracted was filtered, centrifuged and finally processed for biosynthesis of Silver nanoparticles with Silver nitrate. The characterization of AgNPs was confirmed by UV-Vis spectroscopy. The maximum absorbance of Silver nanoparticles synthesized by Parijat leaves were found to be at 425 nm which was then further characterized by X-ray Diffractometer. Furthermore, antimicrobial activity was determined by agar well diffusion method against different human pathogens Escherichia coli, Staphylococcus aureus, Proteus spp. and Salmonella Typhi. The comparison of antimicrobial activity of plants extract and their synthesized nanoparticles concluded that nanoparticles of Supari and Lapsi and Parijat were found to be more effective against test organisms. The study concludes that the nanoparticles biosynthesized from plants products were more effective and demonstrate better antimicrobial activity than that of their extracts. Keywords: Silver nanoparticles, UV–Vis Spectroscopy, X–Ray Diffractometer, Antimicrobial activity, Nepal.

Author Biographies

Sandeep Thapa, College for Professional Studies, Department of Biotechnology, Kathmandu, Nepal
College for Professional Studies, Department of Biotechnology, Kathmandu, Nepal
Rajani Shresth, College for Professional Studies, Department of Biotechnology, Kathmandu, Nepal
College for Professional Studies, Department of Biotechnology, Kathmandu, Nepal
Anjali Tibrewal, College for Professional Studies, Department of Biotechnology, Kathmandu, Nepal
College for Professional Studies, Department of Biotechnology, Kathmandu, Nepal
Mukesh Shrestha, College for Professional Studies, Department of Biotechnology, Kathmandu, Nepal
College for Professional Studies, Department of Biotechnology, Kathmandu, Nepal
Yuvraj K. C., College for Professional Studies, Department of Biotechnology, Kathmandu, Nepal
College for Professional Studies, Department of Biotechnology, Kathmandu, Nepal

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Published
2017-03-28
Section
Article