Biogas production using guinea corn and rice husk
AbstractPreliminary experiment on the possibility of biogas production from guinea corn and rice husks. The two samples (guinea corn and rice husk) were collected from a farm land in Rabah Local Government Area Sokoto, Nigeria. The guinea corn husks were dried and grounded using wooden pestle and mortar. The experimental set up was carried out by measuring 75g each of their pure form and mixture with label A, B, C and D before diluting with water in a ratio of 6:1, 3:1, 1:8 and 1:1 respectively and 10kg of freshly collected rumen content was added to each sample. The total volume of biogas was determined, moisture content, ash content, fiber content and N. P. K values were determined for digested and undigested slurries, as well as bacteriafrom anaerobic and aerobic culture were isolated from the digested slurries. Results indicated that both guinea corn and rice husk produced 1000cm3and 100cm3 of biogas respectively for the period of 28 days with guinea corn having the highest biogas production, the digested slurries were rich in both mineral and nutrient contents and also some methanogenic bacteria andbacteria species identified are P. aeroginosa (11%), Bacillus spp (34%), Staphylococcus spp (16%), Enterococcus spp (9%), Vibrio cholerae (11%) Enterobacter spp (5)Salmonella spp (5%), Morganiella morganii (5%), Lactococcus lactis (2%) and Serratia marcenscens (2%). Guinea corn and rice husk can be used to produce significant amount of biogas, and digested substrates (slurries)can also be used as biofertilizers. Key words: Biogas Production, Bacteriology, Microbial Load, Proximate Analysis, Guinea Corn Husk, Rice Husk, Methanogenic Bacteria.
Abubakar M.M.,Nigerian Journal of Renewable Energy,1990, 1; pp. 69-73.
Aneya, K.R., Experiments in Microbiology, Plants Pathology and Biotechnology, New Age Publishers, ISBIN.2005; p 69.
Arlington, V.A., Bacteriological Analytical Manual, 6th ed., Association of Analytical Chemist for FDA, Washington, D.C. 1992; pp 17-21.
Association of Official Analysis of Chemist (AOAC). Standard Official Methods of Analysis. 15th ed., Washington DC. 1990.
Bagudo, B .U, Garba, B; Dangoggo, S.M and Hassan, L.G. Nigerian Journal of Basic and Applied Science, 2008,16(2); pp 262 – 266.
Baki, A.S., Bello M, Aliyu U and Liman. M.G.Journal of the Chemical Society of Nigeria, 2004, pp.286-288.
Barrow, G.I. and Felthem, R.K.A.Cowan and Steel’s Manual for the Identification of Medical Bacteria. 3rd ed., Cambridge University Press, London.1993.
Cheesbrough, M. District Laboratory Practice in Tropical Countries. Part 2. Low Price Ed., Cambridge University Press, London. 2006.
Collins, C.H and Lyne, P.M. Microbiological Methods, 5th ed., Butterworth and Co. Publ. Ltd, London, 1987, pp. 31-345.
Engler, B., Myles, R and Dhussa, F. Methane Gas Research: Applied Sciences. Publishers Ltd, Essex England. 1995, pp. 15-17.
Garba, B. Journalof World Renewable Energy, 1996, 3; p. 938.
Garba, B. Nigerian Journal of Renewable Energy,1999, 1;pp.12-16.
Garba, B. and Sambo A.S.Nigerain Journal of Renewable Energy, 1992, 3; p. 36.
Garba, B., Uba A., Shehu R.A.Nigeria Journal of Renewable Energy, 2002, 10(1); pp.61-62.
Hansen, K., Optner S.L.,Nyns E.J. Biomethanization., Catholic University Press.Louren, Belgium. 1998, pp. 67-68.
Holt, J.G., Koieg, N.R., Sneath, P.H.A., Starly, J.T., Williams, S.T.Bargey’s Manual of Determinative Bacteriology. 9thedn., Williams and Wikins Press, Boltimore, 1994, p 767.
Misra, N.W., Gunnersosn, C.G and Stuckey, D.R. Integrated Research Recovery – Anaerobic Digestion Principles for Biogas System. World Bank Technical Paper,Genieva,1992, pp.49; 3-4.
Okafor, N. Proceedings of the Conference on Integrated Bio-System, 1998, pp. 1-3.
Okoh, V.C. M.ScDissertification, Usmanu Danfodiyo University, (Sokoto, Nigeria, 1998).
Oluyega, I.O., Femi-Ola, T.O., Opomu, D. Nigerian Journal of Microbiology. 2006, pp. 3: 2-7.
Onyeike, E.N and Osujji, J.O., Research Techniques in Biological and Chemical Science, Springe Field Publishers Ltd. Owerri, Nigeria, 2003.
Rabah, A.B., Baki, A.S., Hassan, L.G., Musa, M. and Ibrahim, A.D. Science World Journal, 2010, 5(4); pp.23 –25.
Samuel, T.D Vincent, F.G, and John, M.S. Experimental Microbiology for the Health Sciences, 4th ed., Burgess Publishing Company, U.S.A, 1979.
Tambuwal, A.D, Dangoggo S.M and Zuru A.A. Nigerian Journal of Renewable Energy, 1997, 5; pp.98 – 100
Wahyudi, A., Hendraningsih, L., Malik, A. African Journal of Biotechnology. 2010, 9(20): pp.2994-2999.
Yadava, L.S and Hesse P.R. The Development and Use of Biogas Technology in Rural Areas of Asia. FAO/UNDP Regional Project Report, 1981.
Zuru, A.A., Abubakar A.B., Yauri, U.A,Birnin-kebbi A.M. Nigerian Journal of Renewable Energy,2001,5(1); pp.50-56.