Purification of keratinase from Aspergillus flavus S125

  • Mini K.D. Dept. of Zoology, Sree Sankara College
  • Kalady k Dept. of Microbiology, School of Biosciences, Mahatma Gandhi University, Kottayam, Kerala
  • Jyothis Mathew Dept. of Microbiology, School of Biosciences, Mahatma Gandhi University, Kottayam, Kerala

Abstract

The aim of this study was to purify a keratinase produced by a newly isolated fungus Aspergillus flavus. The keratinase enzyme was purified by procedures including ammonium sulfate precipitation, ion exchange chromatography and gel filtration chromatography. All these purification steps were performed at 4◦C. After each step keratinase activity and protein content were determined. Keywords: Ammonium sulfate, Aspergillus flavus, Feather, Keratin,

Author Biographies

Mini K.D., Dept. of Zoology, Sree Sankara College
Dept. of Zoology, Sree Sankara College
Kalady k, Dept. of Microbiology, School of Biosciences, Mahatma Gandhi University, Kottayam, Kerala
Dept. of Microbiology, School of Biosciences, Mahatma Gandhi University, Kottayam, Kerala
Jyothis Mathew, Dept. of Microbiology, School of Biosciences, Mahatma Gandhi University, Kottayam, Kerala
Dept. of Microbiology, School of Biosciences, Mahatma Gandhi University, Kottayam, Kerala

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Published
2017-03-28
Section
Article