Purification of keratinase from Aspergillus flavus S125
AbstractThe aim of this study was to purify a keratinase produced by a newly isolated fungus Aspergillus flavus. The keratinase enzyme was purified by procedures including ammonium sulfate precipitation, ion exchange chromatography and gel filtration chromatography. All these purification steps were performed at 4◦C. After each step keratinase activity and protein content were determined. Keywords: Ammonium sulfate, Aspergillus flavus, Feather, Keratin,
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