Significance of direct Gram stained smear examination in sore throat

  • K. Sai leela Kamineni Institute of Medical Sciences, Narketpally
  • G. J. Archana Kamineni Institute of Medical Sciences, Narketpally


The present study was conducted to demonstrate the significance of direct smear examinations of throat swabs collected from sore throat cases. Throat swabs collected from 200 sore throat cases were studied by direct smear examination and culture. Gram positive cocci and Gram negative bacilli were interpreted as probable etiology based on pus cells and predominant organism present in the smear. Culture isolates were corresponding to the interpretation of Gram stain. Out of the 200 isolates, 47 (23%) were culture positive. Among them Gram positive were 24 (12%). The predominant isolate being Streptococcus pneumoniae (25.5%) followed by Staphylococcus aureus (17.02%),Streptococcus pyogenes (6.3%) and Enterococci (4.2%). Gram negative organisms account for 23 (11%). Viruses being the most common etiology in sore throat when compared to bacterial, for which not only the treatment with antibiotics is inappropriate and also the symptomatic treatment is sufficient. Thus Gram stain report helps in reducing the inadvertent use of antibiotic and thereby preventing the emergence of drug resistance. Key words: sore throat, throat swab, Grams stain

Author Biographies

K. Sai leela, Kamineni Institute of Medical Sciences, Narketpally
Kamineni Institute of Medical Sciences, Narketpally
G. J. Archana, Kamineni Institute of Medical Sciences, Narketpally
Kamineni Institute of Medical Sciences, Narketpally


Sloane, P.D., Slatt, L.M., Ebell, M.H. and Jacques, L.B. eds. Sore Throat. Essentials of Family Medicine: Lippincott, Williams and Wilkins, 5th edition, pp. 313-323.

Jerris R. Hedges, David K.Wagner. Journal of the American College of Emergency Physicians. 1978. 7, 6, 3-9, 226-257, 69-88.

C Del Mar. The Medical journal of Australia, 1992, 156, 8, 572-5

S C Sharma, P V Subbukrishnan . Postgraduate Medical Journal, 1981, 57, 13-15

G Crawford · F Brancato. K K Holmes, Annals of Internal Medicine, 1979, 90, 3, 293-297.

James Peter Blackmur, Simon Lammy, David E C Baring. BMJ Case Rep 2013. doi:10.1136/bcr-2013-201977

K. Kranthi Kumar, S.R. Mahesh. Asia J of Microbiol. Biotech. Env. Sc. 2010, 12 (3): 673-676

Ashok Kumar, Kingston Rajiah, International Journal of Pharmacy and Pharmaceutical Sciences, 20124, 4, 181-185

Sore Throat in Young Adults May Indicate Serious Illness.Laurie Barclay, MD, February 18, 2015.

Acute sore throat.Diagnosing ARIs Series,Graham Worrall MB BS MSc FCFP. 2011, Canadian Family Physician, Le Médecin de famille canadien, Vol 57.