In-vitro screening of salinity tolerant rice cultivars at seedling stage and assessment of growth parameters
AbstractRice is a largely cultivated cereal crop for more than a billion people worldwide, which includes twenty million people in Sri Lanka. Salinity has an adverse impact on rice cultivation. Therefore selection of rice cultivars which are tolerant to salinity is an important requirement for paddy cultivation in Sri Lanka. This study was conducted to screen the salinity tolerant cultivar and assess the performance of them under in vitro condition. Fifteen rice cultivars were collected and subjected to different salt concentration. Four treatments were designed as 50, 85, 120 and 155 μs/cm salinity level and control set also maintained with five replications. Seed germination percentage, seedling height and shoot length was measured at 5, 10 and 15 days after planting. Complete randomized design was used to analyze the growth parameters and the mean separated by DMRT. Results showed irrespective of the viability percentage plants germinated relatively at same rate. In case of height of the plant Pothalai cultivars reached maximum at 50μs/cm but it suppressed at high salinity levels. Among tested cultivars Pusparaga and Goda heenati reached maximum height significantly than others while Masuran which maintained in lower height at all salt level. Root length is checked by salt concentration. Even though, Goda heenati and Pushparaga produced high root length at 120μs/cm salinity level. Pothalai, Pusparaga and Goda heenati cultivars can be suggested to salinity affected area while Goda heenati is the best cultivar as it exhibited higher growth performance than the other tested cultivars. Key words: Salinity, cultivar, Goda heenati, germination, concentration
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