Antibacterial potential of actinomycetes from Seagrass against human and aquaculture pathogens

  • M. Babuselvam P. G. & Research Dept. of Zoology, Dr. Zakir Husain College, Ilayangudi – 630 702, Tamil Nadu, India
  • A. Panneerselvam PG and Research Department of Botany and Microbiology A. V. V. M. Sri Pushpam College (Autonomous), Poondi, Thanjavur Dt, Tamil Nadu, India
  • K. Kanimozhi PG and Research Department of Botany and Microbiology A. V. V. M. Sri Pushpam College (Autonomous), Poondi, Thanjavur Dt, Tamil Nadu, India
  • G. Kavitha P. G. & Research Dept. of Zoology, Dr. Zakir Husain College, Ilayangudi – 630 702, Tamil Nadu, India

Abstract

The present investigation was designed to isolate actinomycetes from seagrass and evaluate their antibacterial activity against antibiotic resistant pathogens, urinary tract infections pathogens, ophthalmic pathogens, fish pathogens and shrimp pathogens. A total of 10 actinomycetes species isolated from seagrass namely Cymodocea serrulata and Syrgodium isoetifolium were tested for antagonistic activity against selected clinical and aquaculture pathogens. During the initial screening (primary screening), isolates no. 2,3 and 5 showed inhibition against human pathogens. These three isolates were further screened against antibiotic resistant pathogens, urinary tract infections pathogens, ophthalmic pathogens, fish pathogens and shrimp pathogens. These three isolates showing potent broad spectrum antibacterial activity. The present study also performed that, the cultural, morphological, physiological and biochemical characteristics confirmed that these isolates are species of the genus, Streptomyces. Further studies on the bioactive metabolites from these isolates will be useful for discovering novel compounds of clinical and aquaculture use. Keywords: antibacterial compounds; inhibition; Streptomycetes; seagrass

Author Biographies

M. Babuselvam, P. G. & Research Dept. of Zoology, Dr. Zakir Husain College, Ilayangudi – 630 702, Tamil Nadu, India
P. G. & Research Dept. of Zoology, Dr. Zakir Husain College, Ilayangudi – 630 702, Tamil Nadu, India
A. Panneerselvam, PG and Research Department of Botany and Microbiology A. V. V. M. Sri Pushpam College (Autonomous), Poondi, Thanjavur Dt, Tamil Nadu, India
PG and Research Department of Botany and Microbiology A. V. V. M. Sri Pushpam College (Autonomous), Poondi, Thanjavur Dt, Tamil Nadu, India
K. Kanimozhi, PG and Research Department of Botany and Microbiology A. V. V. M. Sri Pushpam College (Autonomous), Poondi, Thanjavur Dt, Tamil Nadu, India
PG and Research Department of Botany and Microbiology A. V. V. M. Sri Pushpam College (Autonomous), Poondi, Thanjavur Dt, Tamil Nadu, India
G. Kavitha, P. G. & Research Dept. of Zoology, Dr. Zakir Husain College, Ilayangudi – 630 702, Tamil Nadu, India
P. G. & Research Dept. of Zoology, Dr. Zakir Husain College, Ilayangudi – 630 702, Tamil Nadu, India

References

DJ. Faulkner, Natural product reports. 2001;18(1):1R-49R.

H. Weyland, E. Helmke, (Y Okami, ed) Japan Scientif Soc Press, Tokyo. 1988:294-9.

JD,Walker RR.Colwell, Marine Biology. 1975,(3):193-201.

I. Barcina, I., J, Iriberri, L. Egea, Systematic and Applied Microbiology, 1987, 10: 85-91

PR, Jensen R, Dwight W. Fenical, Applied and environmental microbiology. 1991,57(4):1102-8.

ST Williams, S, Lanning EMH Wellington, The biology of actinomycetes. Acdemic, London, 1984, 481–528

SA, Waksman HB.Woodruff, Experimental Biology and Medicine. 1940, 45(2):609-14.

Okami YO, Hotta KU. Search and discovery of new antibiotics.

HP, Fiedler C,Bruntner AT, Bull AC, Ward M, Goodfellow O, Potterat C, Puder G.Mihm Antonie van Leeuwenhoek. 2005, 1:37-42.

PR, Jensen E, Gontang C,Mafnas TJ,Mincer W Fenical, Environ Microbiol, 2005a, 7:1039–1048

J,Ravel MJ, Amoroso RR,Colwell RT. Hill, FEMS microbiology letters. 1998,162(1):177-84.

K. Sivakumar Actinomycetes of an Indian mangrove (Pitchavaram) environment; an inventory Ph.D. Thesis, Annamalai University, Tamil Nadu, 2001.

R. Balagurunathan, A. Subramanian. Adv Biosci. 2001;20(2):71-6.

AD Marderosian. Journal of pharmaceutical sciences. 1969, 1;58(1):1-33.

TF.Molinski, Chemical Reviews. 1993, 93(5):1825-38.

MA. Austin, American journal of epidemiology. 1989,129(2):249-59.

Okami Y., Microbial ecology. 1986 Mar 1;12(1):65-78.

MG, Haygood EW,Schmidt SK,Davidson DJ.Faulkner, Journal of molecular microbiology and biotechnology. 1999, (1):33-43.

I,Raad A,Alrahwan K.Rolston, Clinical infectious diseases. 1998, 26(5):1182-7.

TL,Smith WR.Jarvis, Microbes and infection. 1999,1(10):795-805.

S,Ravikumar S, Nazar A,Nuralshiefa S.Abideen, Journal of environmental biology/Academy of Environmental Biology, 2005 2:383-6.

MJ,Gauthier GN.Flatau, Canadian Journal of Microbiology. 1976, 22(11):1612-9.

JJ,Sanglier H,Haag TA, Huck T.Fehr, Expert opinion on investigational drugs. 1996, 5(2):207-23.

S,Vanajakumar N,Selvakumar R.Natarajan Oxford and IBM Publishing Co Pvt Ltd, New Delhi, Bombay and Calcutta. 1991:289-300.

W. Fenical, PR. Jensen, In Pharmaceutical and Bioactive Natural Products 1993, 419-457). Springer US.

R Patil, G., Jeyasekaran S.A. Shanmugam, and R., Jeya Shakila, Indian J. Mar. Sci., 2001, 30: 264-267.

PR,Jensen W. Fenical, Microbial ecology. 1995, 29(3):249-57.

JA, Trischman DM,Tapiolas PR, Jensen R,Dwight W,Fenical TC,McKee CM,Ireland TJ,Stout J. A Clardy, Journal of the American Chemical Society. 1994,16(2):757-8.

H Weyland. Nature. 1969, 223:858.

Published
2017-03-29
Section
Article