Screening and Isolation of Cellulase Producing Fungal Strains

  • Vijay Kumar Jha Microbial and Molecular Genetics Laboratory, Department of Botany, Patna University, Patna-800005, Bihar, India
  • Birendra Prasad Associate Professor, Microbial and Molecular Genetics Laboratory, Department of Botany, Patna University, Patna-800005, Bihar, India
  • Kumar Pranay Department of Biochemistry, Patna University, Patna- 800005, Bihar, India

Abstract

The aim of this work was to isolate and select fungal strains from soil with the potential to produce enzymes capableof degradation of cellulosic wastes. Twenty-six fungal strains were isolated out of which four were selected for further investigations. Three strains (VBIV, VBVI, and VBIX) were identified as Aspergillus sp. One VBVII was identified as Penicillium sp. All the four isolate showed endoglucanase activity with VBVI showing the greatest activity (0.4427 units). Strains VBVI and VBIX showed better β-glucosidase activity than the other two strains. Exoglucanase activity was highest in VBVI. All the strains showed effective Fpase activity after 72 hrs of incubation. All the strains effectively degraded xylan. The isolated fungal strains showed potential of synthesizing hydrolytic enzymes and could be used to degrade lignocellulosic wastes. Key words: Fungal strains, Aspergillus, Penicillium, hydrolysing enzymes

Author Biographies

Vijay Kumar Jha, Microbial and Molecular Genetics Laboratory, Department of Botany, Patna University, Patna-800005, Bihar, India
Microbial and Molecular Genetics Laboratory, Department of Botany, Patna University, Patna-800005, Bihar, India
Birendra Prasad, Associate Professor, Microbial and Molecular Genetics Laboratory, Department of Botany, Patna University, Patna-800005, Bihar, India
Associate Professor, Microbial and Molecular Genetics Laboratory, Department of Botany, Patna University, Patna-800005, Bihar, India
Kumar Pranay, Department of Biochemistry, Patna University, Patna- 800005, Bihar, India
Department of Biochemistry, Patna University, Patna- 800005, Bihar, India

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Published
2017-03-29
Section
Article