Screening and Isolation of Cellulase Producing Fungal Strains
AbstractThe aim of this work was to isolate and select fungal strains from soil with the potential to produce enzymes capableof degradation of cellulosic wastes. Twenty-six fungal strains were isolated out of which four were selected for further investigations. Three strains (VBIV, VBVI, and VBIX) were identified as Aspergillus sp. One VBVII was identified as Penicillium sp. All the four isolate showed endoglucanase activity with VBVI showing the greatest activity (0.4427 units). Strains VBVI and VBIX showed better β-glucosidase activity than the other two strains. Exoglucanase activity was highest in VBVI. All the strains showed effective Fpase activity after 72 hrs of incubation. All the strains effectively degraded xylan. The isolated fungal strains showed potential of synthesizing hydrolytic enzymes and could be used to degrade lignocellulosic wastes. Key words: Fungal strains, Aspergillus, Penicillium, hydrolysing enzymes
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