Assessment of Biological and Antioxidant Capacities of Myrtuscommunis L. Leaf and Fruit Extracts from Mediterranean Region of Turkey

  • Ayse Gunyakti Department of Biotechnology and Molecular Biology, Faculty of Arts and Sciences, Aksaray University, Aksaray, Turkey
  • Meltem Asan-Ozusaglam Department of Biotechnology and Molecular Biology, Faculty of Arts and Sciences, Aksaray University, Aksaray, Turkey
  • Muhammad Mujtaba Department of Biotechnology, Biotechnology Institute, Ankara University, Ankara, Turkey

Abstract

Recently in humans and fishes most of the disease causing pathogenic microorganisms developed resistance against certain antimicrobial agents. This situation is causing both health and economic problems. In this context present study was designed to evaluate the antimicrobial and antioxidant activities of leaves and fruits of Myrtuscommunis. Antimicrobial activity of the plant’s leaf and fruit extracts was tested by using disc diffusion method against five fish, seven food-borne human pathogens and twoyeast strains. For this purpose, n-hexane and methanol an organic solvent were used for the obtaining the extract from dried leaves and fruit. The highest inhibition values was recorded as; for leaf n-hexane, leaf methanol and fruit n-hexane extracts against A. hydrophila ATCC 19570 fish pathogen as 24.59 mm, 21.76 mm and 23.78 mm, respectively while for fruit methanol extract against E. faecalis ATCC 29212 (human food-borne pathogen) as 16.61 mm. MBC and MFC results revealed that all extracts showed inhibitory activity against the entire test microorganisms. Methanol extracts exhibited higher antioxidant activity than n-hexane extracts. IC50 values were recorded in the range of 162.82-633.01 μg/mL and the highest activity was recorded for methanol extract (162.82 μg/mL). According to total antioxidant capacity method, the leaf methanol extract has been shown to have the highest activity as 115.91 mg AE/g.The highest activity (147.97mg TE/g) was found by the leaf methanol extract using FRAP method. Considering the results M. communis can be suggested as possible candidate for the extraction of natural and economic antimicrobial and antioxidant material.

References

Negi PS. Plant extracts for the control of bacterial growth: Efficacy, stability and safety issues for food application. International Journal of Food Microbiology. 2012;156(1): 7-17.

Chopra, I., and M. Roberts, 2001: Tetracycline antibiotics: mode of action, applications, molecular biology, and epidemiology of bacterial resistance. Microbiol. Mol. Biol. Rev. 20001; 65: 232-260.

Kautsky N, Rönnbäck P, Tedengren M, Troell M. Ecosystem perspectives on management of disease in shrimp pond farming. Aquaculture. 2000;191(1): 145-61.

Kocatepe D, Erkoyuncu İ, Turan H. Su Ürünleri Kaynaklı Patojen Mikroorganizmalar ve Zehirlenmeler. Yunus Araştırma Bülteni. 2013; 3: 47-56.

Akif ER, KAYIŞ Ş. Çay Bitkisi (Camellia sinensis) Tohumunun Gökkuşağı Alabalıklarında (Oncorhynchus mykiss) Aeromonas hydrophila Enfeksiyonuna Karşı Kullanımının Araştırılması. El-Cezeri Journal of Science and Engineering. 2015; 2(3): 67-74.

M. Akin, A. Aktumsek, A. Nostro, Afr. J. Biotechnol., 2010. 9, 531-535.

Aleksic V, Knezevic P. Antimicrobial and antioxidative activity of extracts and essential oils of Myrtus communis L. Microbiological Research. 2014; 169(4): 240-54.

Guidi L, Landi M. Aromatic plants: use and nutraceutical properties. Novel Plant Bioresources: Applications in Food, Medicine and Cosmetics. 2014: 303-45.

Jafari S, Amanlou M, Borhan-mojabi K, Farsam H. Comparartive study of Zataria multiflora and Anthemis nobelis extracts with Myrthus communis preparation in the treatment of recurrent aphthous stomatitis. DARU Journal of Pharmaceutical Sciences. 2003; 11(1): 23-7.

Sumbul S, Ahmad MA, Asif M, Akhtar M. Myrtus communis Linn.-A review. Indian Journal of Natural Products and Resources. 2011; 2(4): 395-402.

Sumbul S, Ahmad MA, Asif M, Saud I, Akhtar M. Evaluation of Myrtus communis Linn. berries (common myrtle) in experimental ulcer models in rats. Human & Experimental Toxicology. 2010; 29(11): 935-44.

D. Djenane, J. Yangüela, T. Amrouche, S. Boubrit, N. Boussad, P. Roncalés, Food Science and Technology International, 2011; 7: 505-515.

Yeğin AB, Uzun Hİ. Phenolic contents of myrtle (Myrtus communis L.) fruits. Derim. 2015; 32(1): 81-8.

Gardeli C, Vassiliki P, Athanasios M, Kibouris T & Komaitis M. Essential oil composition of Pista cialentiscus L. and Myrtus communis L.: Evaluation of antioxidant capacity of methanolic extracts. Food Chemistry, 2008; 107: 1120-1130.

Yildirim H, Kargi SP, Karabiyik Ş. Adana ve Mersin Ekolojik Koşullarında Doğal Olarak Yetişen Mersin (Myrtus communis L.) Bitkileri Üzerinde Bir Araştırma. alatarım. 2013; 12: 1–9.

Avcı, A.B. and E. Bayram. Mersin Bitkisi (Myrtus communis L.) 'nde farklı hasat zamanlarının uçucu yağ oranlarına etkisi. SDU. J. Nat. Appl. Sci., 2008; 12: 178–181.

A. Cakir, Essential oil and fatty acid composition of the fruits of Hippophae rhamnoides L. (Sea Buckthorn) and Myrtus communis L. from Turkey. Biochemical Systematics and Ecology. 2004; 32(9): 809-816.

Kirby AJ, Schmidt RJ. The antioxidant activity of Chinese herbs for eczema and of placebo herbs—I. Journal of Ethnopharmacology. 1997; 56(2): 103-8.

Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Analytical Biochemistry. 1999; 269(2): 337-41.

Aktumsek A, Zengin G, Guler GO, Cakmak YS, Duran A. Antioxidant potentials and anticholinesterase activities of methanolic and aqueous extracts of three endemic Centaurea L. species. Food and Chemical Toxicology. 2013; 55: 290-6.

Mert T, Fafal T, Kivcak B, Ozturk HT. Antimicrobial and cytotoxic activities of Myrtus communis L. J Fac Pharm. 2008; 37(3): 191-9.

Gortzi O, Lalas S, Chinou I, Tsaknis J. Reevaluation of bioactivity and antioxidant activity of Myrtus communis extract before and after encapsulation in liposomes. European Food Research and Technology. 2008; 226(3): 583-90.

Etiz P, Kibar F, Ekenoğlu Y, Yaman A. KAN Kültürlerinden Izole Edilen Candida Türlerinin Dağiliminin Ve Antifungal Duyarliliklarinin Retrospektif Olarak Değerlendirilmesi. Ankem Derg. 2015; 29(3): 105-13.

Ozogul Y, Kuley E, Ucar Y, Ozogul F. Antimicrobial impacts of essential oils on food borne-pathogens. Recent patents on Food, Nutrition & Agriculture. 2015; 7(1): 53-61.

Molyneux P. The use of stable free radical diphenylpicrylhydrazyl (DPPH) for estimating

antioxidant activity. Songklanakarin J. Sci. Technol. 2004; 26 (2): 211-219.

Wannes WA, Mhamdi B, Sriti J, Jemia MB, Ouchikh O, Hamdaoui G, Kchouk ME, Marzouk B. Antioxidant activities of the essential oils and methanol extracts from myrtle (Myrtus communis var. italica L.) leaf, stem and flower. Food and chemical toxicology. 2010; 48(5): 1362-70.

Aidi Wannes W, Marzouk B. Differences between myrtle fruit parts (Myrtus communis var. italica) in phenolics and antioxidant contents. Journal of Food Biochemistry. 2013; 37(5): 585-94.

Sarikurkcu C, Tepe B, Yamac M. Evaluation of the antioxidant activity of four edible mushrooms from the Central Anatolia, Eskisehir–Turkey: Lactarius deterrimus, Suillus collitinus, Boletus edulis, Xerocomus chrysenteron. Bioresource Technology. 2008; 99(14): 6651-5.

Published
2017-06-30
Section
Plant Biotechnology