Physico-chemical and Bacteriological Quality of an Abattoir Wastewater Discharged into Water Bodies in Ibadan, Nigeria and drug resistant profile of isolated Salmonella species
AbstractThe discharge of untreated abattoir wastewater constitutes public health concern because diseases are transmitted through contaminated water bodies. Contaminated water supply systems used for drinking and other purposes have been implicated in the transmission of antibiotic resistant pathogens. This study was carried out to determine the physico-chemical, bacteriological qualities of abattoir wastewater in Ibadan and antibiotics resistance patterns of Salmonella species from the wastewater. Wastewater samples were collected from the slaughter slab and drainage. Physico-chemical parameters, total bacteria counts (TBC) and total coliform counts (TCC) were determined using standard procedures. Salmonella species were isolated from the samples using pour plate techniques and identified using biochemical tests, while susceptibility test of the isolates against 10 antibiotics was determined using disc diffusion technique. The physico-chemical parameters of the wastewater showed total dissolved solids of 4,150 mg/l and 2300 mg/l for slaughter slab and drainage respectively while biochemical oxygen demand was 867.2 mg/l and 698.5 mg/l. Dissolved oxygen was between 0.01 mg/l and 0.02 mg/l; the mean value of TBC and TCC ranged between 4.24x 107- 4.78x107 cfu/ml and 3.03x107-3.51x107cfu/ml respectively. Salmonella species isolated were 48 and were all resistant to ampicillin and ceftriazone. The strains of S. enterica subspecies enterica exhibited resistance to eight antibiotics combination (AMP-TET-OFX-AMC-CLX-CRO-STR-SXT, AMP-TET-AMC-CLX-CRO-CHL-STR-SXT) while two of the isolates were pan-resistant. The abattoir wastewater must be properly treated before being discharged into the environment as it is evident that it harbours multiple antibiotic resistant pathogens.
Amisu K, Coker A, Isokpehi R. Arcobacter butzlieri strains from poultry abattoir effluent in Nigeria. East Africa Medical Journal. 2003; 80: 218-21.
Kosamu, I, Mawenda J, Mapoma H. Water quality change due to abattoir effluent: A case on Mchesa stream in Blantyre, Malawi. African Journal of Environmental science and Technology. 2011; 5(8): 589-94
J Pelczar, E Chan, N Kreig. Microbiology,5th ed., Tata McGraw-Hill, New Delhi 2002.
Osibanjo O, Adie G. Impact of effluent from Bodija abattoir on the physicochemical parameters of Osunkeye stream in Ibadan City, Nigeria. Africa Journal of Biotechnology. 2007; 6 (15): 1806-11.
A Dufour, J Bartram. Animal Waste, Water Quality and Human Health. Bos R & Gannon V. (Eds) IWA Publishing, London 2012.
Ibekwe A, Okonko I, Onunkwo A, Donbraye E, Babalola E, Onoja B. Baseline Salmonella agglutinin titres in apparently healthy freshmen in Awka South Eastern, Nigeria. Science Research Essays 2008; 3 (9): 225-30.
Solomon T, Wudu B, Biruk T, Tesfaye S, Aklilu F. Prevalence and antimicrobial susceptibility pattern of Salmonella species from exotic chicken eggs in Alage, Ziway and Shashemene, Ethiopia. Africa Journal of Basic and Applied Sciences. 2016; 8 (3): 180-4
Marrero-Ortiz R, Han J, Lynne A, David D, Stemper M, Farmer D, Bukhardt W, Bukhardt W, Nayak R, Foley S. Genetic characterization of antimicrobial resistance in Salmonella enterica serovars isolated from dairy cattle in Wisconsin. Food Research International. 2012; 45: 962-7.
Cummings K, Warnick L, Davis M, Eckmann K, Grohn Y, Hoelzer K, MacDonald K, Root T, Siler J, McGuire S, Wiedmann M, Wright E, Zansky S, Besser T. Farm animal contact as risk factor for transmission of bovine associated Salmonella subtypes. Emerging Infectious Diseases. 2012; 18 (12): 1929-36.
Olowe O, Okanlawon B, Olowe R, Adedosu O, Olayemi A. Multiple drug resistant patterns of Salmonella typhimurium infections in Oshogbo: South Western Nigeria. Journal of American Science. 2007; 3 (4): 40-4.
Akinyemi K, Oshundare Y, Oyeyinka O, Coker A. A retrospective study of community-acquired Salmonella infections in patients attending public hospitals in Lagos, Nigeria. Journal of Infections in Developing Country. 2012; 5 (6): 387-95.
Swai E, Schoonman L. A survey of zoonotic diseases in trade cattle slaughtered at Tanga city abattoir: a cause of public health concern. Asian Pacific Journal of Tropical Biomedicine. 2012; 2(1): 55-60.
American Public Health Association (APHA). Standard methods for examination of water and wastewater. American Public Health Association, American Water Works Association and Water Pollution Control Federation. 20th ed., Washington DC, USA,1998; pp.5-17.
Fagade E, Oyelade A, Ogunjobi A, Odumosu M. Incidence of bacteria of public health importance in drinking water from water dispenser systems in homes and offices in Lagos and Ibadan, Nigeria. Internet Journal of Microbiology. 2008; 7 (1): 3-4.
Orji M, Onuigbo H, Mbata T. Isolation of Salmonella from poultry droppings and other environmental sources in Awka, Nigeria. International Journal of Infectious Diseases. 2005; 9: 86-9.
Clinical and Laboratory Standards Institute (CLSI). Performance Standards for Antimicrobial Disk Susceptibility Tests. 9th ed., Document M2-A9; Clinical and Laboratory Standards Institute (CLSI): Wayne, PA, USA 2011.
FEPA (Federal Environmental Protection Agency). National environmental protection regulations (effluent limitation) regulations S.1.8. Federal Republic of Nigeria Official Gazette, 1991; 42: pp.78.
FMENV. National guidelines and standards for water quality in Nigeria. Federal Ministry of Environment, 2011; pp.114.
World Health Organization. Guidelines for drinking water quality. 2004; pp.515.
Nwogu N, Akpor E, Atuanya E. Effluents qualities of government and private abattoirs and their effects on Ikpoba River, Benin City, Edo State, Nigeria. Advances in Biological Research. 2012; 6 (5): 196-201.
Helmer R, Hespanhol I. Water Pollution Control: A Guide to the Use of Water Quality Management Principles. WHO. London, Great Britain. 1991.
Omole D, Longe E. An Assessment of the impact of abattoir effluents on river Illo, Ota, Nigeria. Journal of Environmental Science and Technology. 2008; 1: 56-64.
Nwachukwu M, Akinde S, Udujih O, Nwachukwu I. Effect of abattoir wastes on the population of proteolytic and lipolytic bacteria in a recipient water body (Otamiri River). Global Research Journal of Science. 2011; 1: 40-2.
Saidu M, Musa J. Impact of abattoir effluent on River Landzu, Bida, Nigeria. Journal of Chemical, Biological and Physical Science. 2012; 2(1): 132-6.
Morrison G, Fatoki O, Persson L, Ekberg A. Impact of point source pollution from the Keiskammahoek Sewage Treatment Plant on the Keiskamma River-pH, electrical conductivity, oxygen-demanding substance. Water SA. 2001; 27: 475-80.
kpor O. Wastewater effluent discharge: Effects and treatment processes. International Conference of Chemical, Biological and Environmental Engineering. 2011; 20: 85-91.
Adesemoye A, Opere B, Makinde S. Microbial content of abattoir wastewater and its contaminated soil in Lagos, Nigeria. African Journal of Biotechnology. 2006; 5(20): 1963-8.
Onuoha S, Eluu S, Okata M. In-vitro antimicrobial resistance of Shigella and Salmonella species recovered from abattoir effluent in Afikpo, South Eastern Nigeria. International Journal of Current Microbiology and Applied Sciences. 2016; 5(4): 488-97.
Nafarnda W, Ajayi I, Shawulu J, Kawe M, Omeiza G, Sani N, Tenuche O, Dantong D, Tags S. Bacteriological quality of abattoir effluents discharged into water bodies in Abuja, Nigeria. International Scholarly Research Network ISRN Veterinary Science 2012; Article ID 515689.
Atieno N, Onwuor O, Omwoyo O. Isolation of high antibiotic resistant fecal bacteria indicators, Salmonella and Vibrio species from raw abattoirs sewage in peri-urban locations of Nairobi, Kenya. Greener Journal of Biological Sciences. 2013; 3 (5): 172-8.
Abiade-Paul C, Kene I, Chah K. Occurrence and antibiograms of Salmonellae in effluents from Nsukka municipal abattoir. Nigerian Veterinary Journal. 2003; 1: 48-53.
Reda A, Seyoum B, Yimam J, Andualem G, Fiseha S, Vandeweerd, J. Antibiotic susceptibility patterns of Salmonella and Shigella isolates in Harar, Eastern Ethiopia. Journal of Infectious Diseases and Immunology. 2011; 3(8): 134-9
Garedew L, Hagos Z, Addis Z, Tesfaye R, Zegeye B. Prevalence and antimicrobial susceptibility patterns of Salmonella isolates in association with hygienic status from butcher shops in Gondar town, Ethiopia. Antimicrobial Research and Infections Control. 2015; 4: 21-7
Tsegaye S, Beyene W, Tesfaye B, Tesfaye S, Feleke A. Prevalence and antimicrobial susceptibility pattern of Salmonella species from exotic chicken eggs in Alage, Ziway and Shashemene, Ethiopia. Africa Journal of Basic and Applied Science. 2016; 8 (3): 180-4
Li D, Yu T, Zhang Y, Yang M, Li Z, Liu M, Qi R. Antibiotics resistance characteristics of environmental bacteria from an Oxytetracycline production wastewater treatment plant and the receiving river. Applied Environmental Microbiology. 2010; 76(11): 3444-51.
Beyene T, Yibeltie H, Chebo B, Abunna F, Beyi A, Mammo B, Ayana D, Duguma R. Identification and antimicrobial susceptibility profile of Salmonella isolated from selected dairy farms, abattoir and humans at Asella Town, Ethiopia. Journal of Veterinary and Science Technology. 2016; 7: 320-4
Ogidi C, Oyetayo V. Antibiotic sensitivity pattern of microorganisms isolated from remnant foods and wastewater from restaurants. FUTA Journal Research in Sciences. 2013; 2: 209-16.
Adley C, Dillon C, Morris CP, Delappe N, Cormican M. Prevalence of Salmonella in pig ear pet treats. Food Research International. 2011; 44: 193–7.
Singh S, Yadav A, Singh S, Bharti P. Prevalence of Salmonella in chicken eggs collected from poultry farms and marketing channels and their antimicrobial resistance. Food Research International. 2010; 43: 2027–30.
Reisenfeld C, Goodman R, Handelsman J. Uncultured soil bacteria are a reservoir of new antibiotic resistance genes. Environmental Microbiology. 2004; 6: 981-9.
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